Technical details
| Product | Deoxynivalenol DON ELISA Kit |
|---|---|
| Item # | F1003 |
| Principle | Competitive ELISA |
| Detection Limit | 0.1 ng/mL |
| Sample Applications | Feed, cereal, |
| Package Content | Precoated microwell, 96wells Deoxynivalenol (vomitoxin, DON) standard series, 0.1 - 8.1 ng/ml Enzyme conjugate TMB Substrate Stop solution (not available when shipping by air) Sample buffer Wash buffer |
| Storage | 2-8 ℃ in cool dark place |
| Shelf Life | 12 months when stored properly |
| Validation | Internal validation |
| Application Notes | This product is validated for milk, milk powder samples. Other sample application may be provided upon request. The product is intended for research use purpose. |
Vomitoxin, also known as deoxynivalenol, is a type B trichothecene, an epoxy-sesquiterpenoid. This mycotoxin occurs predominantly in grains such as wheat, barley, oats, rye, and corn, and less often in rice, sorghum, and triticale.
This Deoxynivalenol (vomitoxin, DON) ELISA Kit is based on indirect competitive ELISA, the coupled antigen was pre-coated in the microwells, the zearalenone will compete for the antibody with the pre-coated antigen, then add HRP enzyme conjugate and TMB substrate successively, the OD value of the sample is negatively correlated with the content of zearalenone contained in the sample. The standard curve is fitted by regression and multiplied by the corresponding dilution ratio to obtain the content of zearalenone in the sample.
This product is a enzyme linked immunosorbent assay to detect deoxynivalenol in feed, cereal etc. It is for quantitative detection of deoxynivalenol in feed and cereal. It is a perfect replacement for HPLC analysis.
This kit is validated internally and externally according to ISO standards.
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