Technical details
| Product | Streptomycin ELISA Kit |
|---|---|
| Item # | E6204 |
| Principle | Lateral flow assay |
| Detection Limit | 0.2 ng/mL |
| Sample Applications | Milk, milk powder, honey, and other biological samples |
| Package Content | Precoated microwell, 96wells Streptomycin standard series, 0.2 - 51.2 ng/ml Enzyme conjugate TMB Substrate Sample buffer Wash buffer Stop solution (2M sulfuric acid) not available when shipping by air |
| Storage | 2-8 ℃ in cool dark place |
| Shelf Life | 12 months when stored properly |
| Validation | Internal validation |
| Application Notes | This product is validated for milk, milk powder and honey samples. Other sample application may be provided upon request. The product is intended for research use purpose. |
Streptomycin ELISA Kit is a competitive ELISA kit to detect Streptomycin and dihydrostreptomycin in milk, milk powder, honey and cell culture samples.
Streptomycin is an aminoglycoside antibiotic used in apiculture to protect bees against a variety of brood diseases. In veterinary practice, it is used to treat bacterial diseases in cattle, pigs, sheep, and poultry. Brazilian authorities have included it in the National Regulatory Monitoring Program for honey production.
This current Streptomycin ELISA Kit is based on indirect competitive ELISA to detect Streptomycin residue in cell culture, milk, and honey. In the kit, streptomycin antigen is coated on the microtiter well and samples contained the drug will compete for streptomycin specific antibody with the antigen. After washing and adding the HRP conjugate, TMB substrate is used to show the reaction color. The concentration of streptomycin in sample is inversely proportional to the OD value, which can be interpreted with a standard curve.
Further readings on Streptomycin and Streptomycin testing:
- Granja, R. H., Niño, A. M. M., Zucchetti, R. A., Niño, R. E. M., Patel, R., & Salerno, A. G. (2009). Determination of streptomycin residues in honey by liquid chromatography–tandem mass spectrometry. Analytica chimica acta, 637(1-2), 64-67.
- Baxter, G. A., Ferguson, J. P., O’Conno, M. C., & Elliott, C. T. (2001). Detection of streptomycin residues in whole milk using an optical immunobiosensor. Journal of agricultural and food chemistry, 49(7), 3204-3207.
