Technical details
| Product | Sulfonamides ELISA Kit |
|---|---|
| Item # | E6203 |
| Principle | Competitive ELISA |
| Detection Limit | 0.2 ng/mL |
| Sample Applications | Milk, milk powder, honey, and other biological samples |
| Package Content | Precoated microwell, 96wells Sulfonamides standard series, 0.2 - 51.2 ng/ml Enzyme conjugate TMB Substrate Stop solution (not available when shipping by air) Sample buffer Wash buffer |
| Storage | 2-8 ℃ in cool dark place |
| Shelf Life | 12 months when stored properly |
| Validation | Internal validation |
| Application Notes | This product is validated for milk, milk powder samples. Other sample application may be provided upon request. The product is intended for research use purpose. |
Sulfonamides represent a large class of antibiotics that have multiple clinical uses. They are considered bacteriostatic and appear to act by inhibition of bacterial biosynthesis of folic acid, which is needed for cell growth, at least in those bacteria that are sensitive to sulfonamides. Now in all major countries, strict maximum residue limits have been set on Sulfonamides. In EU, the total residue of sulfonamides is set at 100ug/kg for milk, meat, etc.
This current Sulfonamides ELISA Kit is based on indirect competitive ELISA to detect sulfonamides residue in milk and honey. In the kit, sulfa antigen is coated on the microtiter well and samples contained the drug will compete for Sulfonamides specific antibody with the antigen. After washing and adding the HRP conjugate, TMB substrate is used to show the reaction color. The concentration of sulfonamides in sample is inversely proportional to the OD value, which can be interpreted with a standard curve.
This Sulfonamides ELISA kit is based on competitive ELISA to detect sulfonamides in milk, milk powder, honey, cell culture and other biological samples.
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